The Biochrom 20 protein hydrolysate systems analyse the blocked lysine after hydrolysis of the DNP derivatised feed sample. The difference between this figure and that for total lysine in the sample gives the available lysine.
If this does not answer your question or solve your problem, then please contact us at support@biochrom.co.uk detailing your question or the problem you are experiencing. Please list if relevant the instrument, software and version numbers you are running, and enclose any relevant data files, etc.