Please click on the
appropriate Error Code below to display a description, with possible cause,
remedy and solution regarding the error code you are interested in.
|
Description |
Possible Cause |
Remedy And Symptom |
|
No communication with the
Autosampler |
Autosampler not in serial
mode |
Check the Autosampler is in serial mode, and that the serial cable is connected properly. |
|
Description |
Possible Cause |
Remedy And Symptom |
|
Reaction coil over
temperature. |
Control circuitry failed. |
Switch off instrument. Call service engineer. |
|
Description |
Possible Cause |
Remedy And Symptom |
|
Photometer not lit. |
a) Lamp not in position. |
a) Install lamp lamp correctly. |
|
b) Lamp failed. |
b) Replace lamp. |
|
Description |
Possible Cause |
Remedy And Symptom |
|
High coil pressure. |
a) Blockage of reaction
coil, flowcell or back pressure valve. |
a) Isolate area of blockage and clean. |
|
b) Reaction coil cold. |
b) Set correct reaction coil temperature. |
|
|
c) Blockage in drain pipe. |
c) Disconnect and flush line. |
|
Description |
Possible Cause |
Remedy And Symptom |
|
High Ninhydrin
Pressure |
a) Ninhydrin
flow rate too high. |
a) Set flow rate to correct value. |
|
b) Ninhydrin
back pressure valve blocked. |
b) Strip and clean the valve, change O ring. |
|
|
c) Blocked pipe at inlet
to mixing T. |
c) Remove and check pipe. |
|
Description |
Possible Cause |
Remedy And Symptom |
|
High buffer pressure. |
a) Column inlet frit dirty. |
a) Change frit. |
|
b) Column temperature too
low. |
b) Check programming and check heating is functioning. |
|
|
c) Resin contaminated. |
c) Clean resin and repack column. |
|
|
d) Prewash
column blocked. |
d) Change inlet frit. |
|
|
e) Buffer flow rate too
high. |
e) Set flow rate to correct value. |
|
|
f) Capsule or autoloader
blocked. |
f) Unload and remove capsule, check autoloader. |
|
|
g) Restriction in column
inlet pipe. |
g) Remake column inlet pipe connection. |
|
Description |
Possible Cause |
Remedy And Symptom |
|
Low coil pressure. |
a) Leak in fluidics. |
a) Check for leaks. Remake or tighten fittings as required. |
|
b) Coil back pressure
valve faulty. |
b) Examine and clean valve. |
|
|
c) Air in coil pressure
transducer. |
c) Bleed and prime pressure transducer. |
|
Description |
Possible Cause |
Remedy And Symptom |
|
Low ninhydrin
pressure. |
a) Ninhydrin
reservoir empty. |
a) Refill ninhydrin reservoir |
|
b) Flow through ninhydrin bottle filter restricted. |
b) Clean or replace filter |
|
|
c) Air in ninhydrin pump. |
c) Bleed ninhydrin pump |
|
|
d) Air in ninhydrin pressure transducer. |
d) Bleed and prime pressure damper coil with distilled water |
|
|
e) Ninhydrin
pump vernier set too low. |
e) Change to correct setting |
|
|
f) Leak in ninhydrin fluidics. |
f) Check for leaks. Remake or tighten fittings as required |
|
|
g) Dirt in check valves. |
g) Clean valves |
|
|
h) Pump sapphire plunger
broken. |
h) Replace plunger |
|
|
i) Pump motor stopped. |
i) Check programming. If pump faulty, call service engineer |
|
|
j) Pump seal leaking. |
j) Tighten seal or replace if necessary |
|
|
k) Diverter valve open to
DRAIN. |
k) Close valve. |
|
Description |
Possible Cause |
Remedy And Symptom |
|
Low buffer pressure. |
a) Air in pump. |
a) Check buffer reservoir. Refill and prime as required. Bleed pump. |
|
b) Leak in buffer fluidics
prior to column. |
b) Check for leaks. Remake or tighten fittings as required. |
|
|
c) Diverter valve set to
DRAIN. |
c) Set valve to COLUMN. |
|
|
d) Air in buffer pressure
transducer damper. |
d) Bleed and prime pressure damper coil with distilled water. |
|
|
e) Buffer pump vernier set too low. |
e) Change to correct setting. |
|
|
f) Dirt in check valves. |
f) Clean valves. |
|
|
g) Buffer solenoid not
operating. |
g) Check that valve clicks when operated. Call service engineer if valve faulty. |
|
|
h) Buffer pump sapphire
plunger broken. |
h) Replace plunger. |
|
|
i) Pump motor stopped. |
i) Check programming. Call service engineer if pump faulty. |
|
|
j) Pump seal leaking. |
j) Tighten seal or replace if necessary. |
|
Description |
Possible Cause |
Remedy And Symptom |
|
Low nitrogen |
a) Cylinder empty. |
a) Replace cylinder. |
|
b) Regulator faulty or
incorrectly set. |
b) Check regulator. |
|
|
c) Large nitrogen leak. |
c) Trace and repair connection. |
|
Description |
Possible Cause |
Remedy And Symptom |
|
Column over temperature. |
a) Column heating control circuit
faulty. |
a) Call service engineer. |
|
b) Peltier
element faulty. |
b) Call service engineer. |
|
Description |
Possible Cause |
Remedy And Symptom |
|
Ninhydrin pressure higher than buffer pressure. |
Buffer pump failed but did
not shutdown on ERROR 8. |
See ERROR 8. |
|
Description |
Possible Cause |
Remedy And Symptom |
|
Bottles level low (active
when Reagent management system has been activated). |
a) Bottle levels Low. |
a) Top up bottles. |
|
b) Bottle levels were not
updated when the bottles were filled last time. |
b) Reset the levels of reagents in the software to the correct values of the bottles. |
If this does not answer your question or solve your problem, then please contact us at support@biochrom.co.uk detailing your question or the problem you are experiencing. Please list if relevant the instrument, software and version numbers you are running, and enclose any relevant data files, etc.