Frequently Asked Questions

Amino Acid Analysis

Question

Can you tell me the standard procedure in using Internal Standard in the sample preparation?

Answer

To use the internal standard norleucine (Nleu) for calculation make up a stock solution at 50nmol/20µL and add 1 volume of this solution to 4 volumes of your amino acid calibration standard to give a final concentration of 10nmol Nleu/20µL of standard loaded.

For sample preparation, add the Norleucine stock solution to your sulphosalicylic acid deproteinisation solution to give a final loaded amount of 10nmol Nleu/20µL.

To calculate amino acids following preparation and check for sample losses, select the internal standard report option in your method.


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