Frequently Asked Questions

Microplate Instrumentation

Question

What are common wavelengths used for absorbance measurements in the visible range?

Answer

Detection Abbreviation Wavelength Measurement (nm)
ELISA    

p-nitrophenyl phosphate

PNPP 405

3,3′,5,5′-tetramethylbenzidine

TMB 450

o-Phenylenediamine

OPD 492

2,2'-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid

ABTS 405

Nicotinamide adenine dinucleotide

NADH/NADPH 492

5-bromo-4-chloro-3-indoxyl phosphate

BCIP 405

Cell Proliferation

   

3-(4, 5-dimethylthiazolyl-2) -2, 5-diphenyltetrazolium bromide

MTT 570 or 540

sodium 2,3,-bis(2-methoxy-4- nitro-5-sulfophenyl)-5-[(phenylamino)-carbonyl]-2H-tetrazolium

XTT 490 - 500

Bromodeoxyuridine

BrdU 450
Protein    
Bradford   595
BCA   562
Lowry   650

Modified Lowry

  750

Biuret

  540

Pierce Protein Assay

  660

Misc

   

Phosphate

  690

Blue Dextran

  690


Feedback

0 rating from 0 votes

Your feedback is important to us. Why not take a moment to let us know how helpful you found our answer by using the star-rating.



Back to question list