Help
ELISA: An enzyme-linked immune-absorbent assay (ELISA) is an immunodetection assay which allows for identification of specific antigens in samples. Horseradish peroxidise (HRP) is typically conjugated to the secondary antibody to detect the binding of antibody to antigen of interest. Chromogenic substrate for HRP such as TMB (tetramethybenzidine hydrochloride) can be use as a detection reagent. TMB absorbs visible light at 450 nm. All of Biochrom's Anthos and Asys microplate readers, measure light absorbtion at 450 nm.
Cell Density: an important aspect of preparing bacterial cultures is to determine the growth phase before harvesting the cells. Measurement at 600 nm allows for this determination.
DNA and RNA absorb light efficiently in the UV range. Quantitation of nucleic acid occurs at 260 nm. Purity can be determined by measuring both at 260 nm and 280 nm as protein absorbs at 280 nm. Protein is a common contaminate in DNA and RNA preparations.
Kinetics: Enzyme kinetics is an important aspect of enzymology. The use of a chromagenic substrate allows for the determination of enzyme kinetics such as the Michaelis-Menten constant and the rate of a reaction.
NADH: Nicotinamide Adenine Dinucleotide (NAD) is an important co-enzyme that is critical for numerous metabolic pathways. The reduced form of NAD, NADH has a distinct absorbance peak at 340 nm which allow for the calculation of the amount of NADH present in a solution.
Protein Assays: A variety of commercially available kits allow for measuring the amount of total protein in a sample. Reagents are typically used that change color linearly in response to the amount of protein in the sample. Commonly used assays include: BCA, Lowry and Bradford.
Wavescan: Also known as spectral scanning or wavescan measurements. The absorbance of a sample is measured over a specified wavelength range. The position and magnitude of these peaks and valleys will give an indication of sample composition and purity.
Well Scanning: Is a series of measurements within a single well of a microplate used to evaluated wells with an uneven composition such as in agglutination assays and formation of precipitates.
Still Stuck?
Skip this question or contact our technical support team for help
